Histopathological Effects of Carbofuran on Renal and Hepatic Tissues of Albino Mice (Mus musculus).
Wide spread use of pesticide in public health programme and agriculture has caused a severe environmental pollution and potential health hazards. Among all the pesticides used, carbamates are most common. The carbofuran (2, 3-dihydro-2,2-dimethyl-7- benzofuranyl) is an important organocarbamate pesticide. It is a broad spectrum, non- cumulative carbamate insecticide; a cholinesterase inhibitor with contact and stomach action and highly toxic to mammals.
The present studies was undertaken to determine the histopathological effects of carbofuran on renal and hepatic tissue of Albino mice. The study was conducted on male mice only, converting two groups, i.e. one control and one treatment group. The control group consists of 4 mice supplied with standard suggested food and the treatment group consists of 16 mice which were then subdivided into 4 groups (T1, T2, T3, and T4). These groups of animals were orally administered carbofuran at doses of
0.5, 1.0, 1.5, 2.0 mg per body weight per day respectively with food supplements. One of the 4 mice from each group was sacrificed after 15 days, 25 days, 35 days and 45 days.
Histological micrograph of the tissues of the control showed no abnormalities in structure, color, appearance. But the treatment group showed considerable changes compared to control. In case of kidney section after 15 days showed oedema, dialated tubules, congested glomeruli, widen urinary space, vacuolated tubules inflammatory cellular infiltration, which was become more severe in the higher doses with haemorrhage, necrosis, increased number of monocytes and podocytes. In case of liver section, shrinked portal vein, fibrosis in the portal vein, iron material deposited large and dark hepatocytes and kupffer cells (called hemosiderine cells), haernorrhage; dialated sinusoid have been shown after 15 days of treatment. In the higher doses, dialated central vein, congestion has been found with the other changes previously mentioned.
Results obtained from the present investigation clearly indicated that, carbofuran has strong toxic effects on histopathology of renal and hepatic tissues.